16 Feb 2016 Open the CST experiment template from the Flow Lab folder. Press the RUN and LOW buttons on the instrument. Click “Acquire Data” on the

BD FACSDiva Software Reference Manual. Simpler Gating Features † Proportional resizing of polygon and rectangular gates. See Editing Gates on page 48 in this guide. † Drag and drop gates within a plot or from one plot to another within a worksheet. See Dragging and Dropping Gates into Plots on page 232 in the BD FACSDiva Software Reference

Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first. Be aware that the lasers take about 15 minutes to warm up and BD LSR Fortessa X20 – How to Use Guide 2 Section 1: Instrument Startup 1 The instrument is: OFF (go to step 2); ON (go to step 4). 2 Turn ON the cytometer pressing the green button on its right side. Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first.

Fortessa bd manual

BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container.

Essentially each door handle is guaranteed for 100,000 revolutions.

Pages 1/4 Fortessa - Troubleshooting November 2018 General note Please report all issues that you could solve or not solve using our feedback form. If you cannot eliminate the problem independently try to call one of the emergency numbers. Questions / reporting technical problems: Emergencies: Feedback form .

A1). b. Access this manual from the BD FACSDiva Software Help menu (Help > Literature > Reference Manual), or by double-clicking the shortcut on the desktop.

BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30

TM Instructions: • Install the equipment by carefully following the instructions given by the manufacturer and in compliance with the legislation in force. • All the equipment must only be used for the purpose it was built for Fortessa declines any responsibility for improper use of … Flow Cytometry Service BD LSR Fortessa X20 – How to Use Guide 5 13 Install the unstained control sample in the cytometer and set the speed to LOW. Start acquisition in the Acquisition Dashboard.

BD FACSymphony™ A5. Flow Cytometers User's Guide BD FACS LSRFortessa HTS Operation Manual. 1 (Please refer to LSR Fortessa Standard Operation Protocol – Basic operation for Tube.
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• Select a well or group of wells, and then click the Add Setup Controls button . • Select a well or group of wells, and then click the Add Specimen Wells button . • The loader (HTS) settings can be modified for the selected well or plate.

BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes. Table of lasers and filters available: Laser LSR Fortessa manual; For training on the LSRFortessa please contact View and Download fortessa FTK5 instructions manual online.
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form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without the prior written permission of BD Biosciences, 2350

Click “Acquire Data” on the Sample. Injection.

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See the BD CBA Software User's Guide for details. d. BD CaliBRITE™ 3 Beads ( Cat. No. 340486). Unless otherwise specified, all products are

5 Select Tube_001 (inside the syringe) by clicking in the green arrow. 6 Do 3 PRIMEs with the SIP 2 arm open and no tube installed. Then set the cytometer to RUN. 7 Acquire and record dH 2O for 3 minutes in HIGH (name it ‘Initial’).