Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II. Mischo H.E., Hemmerich P., Grosse F., Zhang S. Formation of gamma-H2AX foci is a P. O.cellular response to genotoxic stress, such as DNA double strand breaks or stalled replication forks.

tvärkompletterande protein 6 (XRCC6, även känd som Ku70), förutsagd som UniProt Q9UFW8) hade starka DNA- och C- och G-rika nukleotidpreferenser 

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information. Q23976; UniProt/TrEMBL Ku70, bridge and pillars Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. ku70/80 ku7000 ku70 antibody ku70 uniprot ku7000 specs ku70/80 function ku70ut7000fxkr ku70ut7080fxkr ku70 and ku80 function Please note that this site uses cookies to personalise content and adverts, to provide social media features, and to analyse web traffic. Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders.

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We used the amino acid sequences of Ku70 orthologs from S. cerevisiae (UniProt ID PLA0263 Sigma-Aldrich Rabbit anti-Ku70 Antibody, Affinity Purified Powered by Bethyl Laboratories, Inc. Synonym: 5′-dRP lyase Ku70, 5′-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, 70 kDa subunit, 70kDa, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, CTC box binding factor 75 kDa subunit, CTC box KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource. 2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance.

Alternative Names, 5''-deoxyribose- 5-phosphate lyase Ku70 antibody; 5''-dRP lyase Ku70 antibody; 70 kDa subunit  annotation (IEA) methods that are supplied by the UniProt-GOA project to the GO Consortium: InterPro, IPR006164, Ku70/Ku80 beta-barrel domain. InterPro   Ku70 has been referred to by several names including: Lupus Ku autoantigen protein p70; ATP-dependent DNA helicase 2 subunit 1; X-ray repair complementing  X-ray repair cross-complementing protein 6 (KU70).

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini.

P12956. Description. General description.

The precise role of the Ku70 gene in this process has not yet been determined, in part because no cell lines, animals, or human diseases involved with deficiencies in this gene have yet been identified. Both the human and the mouse Ku70 cDNAs have been cloned, and the human gene has been mapped to chromosome 22q13.

Gene Synonyms. D22S671, D22S731, G22P1, KU70, ML8. Location. Recombinant Human Ku70 protein is a Wheat germ Full length protein 1 to 609 aa range and validated in WB, ELISA, SDS-PAGE. The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the UNIPROT ALIGN P12956. N P E G K V  Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non- homologous end joining (NHEJ) required for double-strand break repair. Oct 6, 2020 4941, Sequence mapping, 1, The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70  The C-terminal region consists of a 5 kDa SAP domain (Ku70-SAP) which involved in DNA binding during NHEJ reaction.

The Ku proteins have previously been shown to bind to breaks in DNA where they function to recruit additional proteins that promote DNA repair. Interestingly, Ku70 and Ku 86 negatively regulate the transcriptional activity of ESE-1. The Ku70/XRCC6 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Ku70/XRCC6 protein expression profile in cells. The kit can be used for measuring the relative amounts of Ku70/XRCC6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Ku70/XRCC6. Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig.
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Description. General description. Lupus Ku autoantigen protein p70 (Ku70) is also known as X-  Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818.

Involved in non-homologous end joining (NHEJ) DNA double strand break repair.
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Jan 1, 2017 Single-strand DNA-dependent helicase of the KU70/KU80 complex participates in the repair of UniProt: the universal protein knowledgebase.

a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig. a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86. The blocking of the Ku70 and Ku86 interaction by HSF1 induced defective NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in Ku70 or Ku80 Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247.